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. 2014 Jul 18;9(7):e101200. doi: 10.1371/journal.pone.0101200

Figure 6. Adherence and invasion of UPEC CFT073 to mouse bladder urothelium tissue.

Figure 6

(A) Quantitative comparison of adherence of CFT073, CFT073 ΔecpA, CFT073 ΔecpA (pMAT9) in DMEM at 37°C. (B) The gentamicin-protection (Invasion) assay was done to quantify the ability of UPEC strains to invade bladder urothelium from infant mice ex vivo. The difference in invasion between CFT073 vs. CFT073 ΔecpA is considered to be statistically significant (p<0.0001). For panels A and B standard deviations are represented by error bars and represent the average of all the results obtained from the 3 experiments performed. (C) Histological sections of mice bladder urothelium infected ex vivo with bacteria of CFT073 wild-type, CFT073 ΔecpA and CFT073 ΔecpA (pMAT9) stained with Hematoxylin-Eosin. The arrows point to the bacteria present. Few to none bacteria were seen in the mutant and mock-infected tissues while numerous wild-type and complemented strain bacteria can be seen throughout the tissue. Images were taken with a 60× objective.