Table 1. Bacterial strains and plasmids used in this study.
Strains | Notes | Reference |
CFT073 | Clinical isolate | [35] |
CFT073ΔecpA | Wild-type strain mutated in ecpA::km | This study |
CFT073ΔecpA (pMAT9) | ΔecpA complemented with pMAT9 | This study |
F11 | Clinical isolate | [36] |
F11ΔecpA | Wild-type strain mutated in ecpA::km | This study |
F11ΔecpA(pMAT9) | ΔecpA complemented with pMAT9 | This study |
UTI89 | Clinical isolate | [37] |
Plasmids | ||
pKD46 | Red recombinase system plasmid | [27] |
pKD4 | Kanamycin cassette | [27] |
pMAT9 | ecpAB (matBC) in pSE380 | [14] |