Table 2. Primers used in this study.
Name | Sequence | Use |
G60 | GTTCTGGCAATAGCTCTGGTAACGGTGTTTACCGGCGTGTAGGCTGGAGCTGCTTC | Mutagenesis |
G61 | TTAACTGGTCCAGGTCGCGTCGAACTGTACGCTAACCATATGAATATCCTCCTTAG | Mutagenesis |
G90 | AACAGCAATATTAGGGGCGTG | Screening mutants |
G91 | GGATAACAGCAGAGCGAGAAG | Screening mutants |
K1 | GCCCAGTCATAGCCGAATAGCCT | Screening mutants |
K2 | CGGTGCCCTGAATGAACTGCAGG | Screening mutants |