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. 2014 Jul 18;9(7):e100985. doi: 10.1371/journal.pone.0100985

Figure 5. Cholesterol induces PI3K-Akt activation with no inflammatory response.

Figure 5

ARPE19 cells were treated with 8 µM Ch or 7KCh for 6 hr. (a) Immunoblot demonstrating significant phosphorylation of Akt by both treatments. (b) qRT-PCR measurements of the inflammatory markers (mean ± s.d., n = 3) after treatment with 8 µM Ch and 7KCh for 24 hr. Cholesterol causes significant phopsphorylation of Akt but no significant inflammatory response. This is additional evidence indicating that PI3K-Akt signaling is not involved in the 7KCh-induced inflammatory response. *p<0.05, two-tailed Student's t-test.