Figure 3. HIM-6 is a 3'-5' DNA helicase.
(A) 3'-ssDNA tailed duplex, (B) 5'-ssDNA tailed duplex, and (C) blunt-ended duplex substrates. Helicase reactions were performed with the indicated concentrations of HIM-6 and 1 nM substrates. Lane 1, no enzyme control; lanes 2-4, HIM-6 5 nM, 10 nM, and 20 nM, respectively; lane 5, heat denatured DNA substrate (* indicates the 5'-end labeled with 3 2P-label). (D) Quantitation of the data from panels D-F expressed as relative percentages of unwinding. Data are means of at least 3 independent experiments ± SD: (○) 3'-ssDNA tailed duplex; (□) 5'-ssDNA tailed duplex; (▵) blunt-ended duplex.