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(A) Helicase reactions with the indicated concentrations of HIM-6 and 1 nM forked duplex substrate lengths (19–34 bp). Lane 1, no enzyme; lanes 2–6, HIM-6 3.25 nM, 6.5 nM, 13 nM, 26 nM, and 52 nM, respectively; lane 7, heat-denatured DNA substrate. (B) Quantification of the data, expressed as means of at least 3 independent experiments ± SD: (•) 19 bp, (▪) 22 bp, (○) 28 bp, (▵) 34 bp.
