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Unwinding of D-loop (A) and HJ (B) structures. Helicase reactions were performed with the indicated concentrations of HIM-6 and 1 nM substrates. Lane 1, no enzyme; lanes 2-5, HIM-6 2.5 nM, 5 nM, 10 nM, and 20 nM, respectively; lane 6, heat-denatured DNA substrate.
