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. 2014 Jul 18;9(7):e102569. doi: 10.1371/journal.pone.0102569

Figure 1. High-throughput sorting of the highest protein-productive cell by a transiently protein-anchored system.

Figure 1

(A) Strategy and organization of the transiently protein-anchored system. (B) Schematic representation of the gene construction of the transiently protein-anchored system using anti-EGFR antibody as an example. The construction includes, from N to C termini, an immunoglobulin leader sequence (LS), an HA epitope, the anti-EGFR antibody fragment, the furin cleavage site (RAKR), and the immunoglobulin C2-type extracellular-transmembrane-cytosolic domains of murin B7-1 antigen (B7).