Figure 4. Lik1 mutant plants are more resistant to Pseudomonas syringae pv. tomato DC3000.

A. Ten-day-old mutant and wild-type seedlings were inoculated with P. syringae pv. Tomato DC3000 at the concentration of 0.5×10⁴ cfu/ml by soaking in a bacterial suspension for 3 hours. The bacterial solution was then removed and the seedlings were washed three times with H₂O prior to incubation. Bacterial growth was measured by grinding the seedlings and then plating the resulting extracts on YPD medium with rifampicin and kanamycin as selection. The data are shown as the log10 of colony forming units (3 hours and 48 hours after inoculation) per seedling. The data are the average of 18 seedlings. Bars represent standard deviations. Student T-test (**) P<0.01. The experiment was done in triplicate, each with similar results. B. Leaf populations of P. syringae pv. tomato strain DC3000 from 4-week-old. Data are mean ±SE for three separate experiments. Student T-test (*) P<0.05.