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. 2014 Jul 18;9(7):e102542. doi: 10.1371/journal.pone.0102542

Figure 2. Gene expression of Vegf-A and Mcp-1 by qRT-PCR at the outflow vein of AVF three days after fistula placement in Iex-1 KO and WT mice.

Figure 2

Immunohistochemical staining for VEGF-A, MCP-1, and MMP-9 at twenty-eight days after AVF placement in Iex-1 KO and WT mice. a. Pooled data for Vegf-A expression by qRT-PCR in outflow veins of AVF three days after fistula placement. There is a significant decrease in the average gene expression of Vegf-A in the Iex-1 KO animals when compared to WT controls (P<0.05). b. Representative staining for VEGF-A on sections removed from the outflow vein of Iex-1 KO (second column) and WT controls (third column) is shown. IgG antibody staining was performed to serve as negative control in the first column. Brown staining cells are positive for VEGF-A. c. Pooled data from the semiquantitative analysis for intensity of VEGF-A staining in the vessel wall of the outflow vein specimens removed from Iex-1 KO and WT mice. There is a significant decrease in the mean VEGF-A staining in the Iex-1 KO animals when compared to WT controls (P<0.05). d. Pooled data for the average gene expression of Mcp-1 by qRT-PCR in outflow veins of AVF three days after fistula placement. There is a significant decrease in the expression of MCP-1 in the Iex-1 KO animals when compared to WT controls (P<0.05). e. Representative staining for MCP-1 on sections on the outflow vein removed from Iex-1 KO (second column) and WT controls (third column) are shown. IgG antibody staining was performed to serve as negative control in the first column. Brown staining cells are positive for MCP-1. f. Pooled data from the semiquantitative analysis for intensity of MCP-1 staining in the vessel wall of the outflow vein specimens removed from Iex-1 KO and WT mice. There is a significant decrease in the mean MCP-1 staining in the Iex-1 KO animals when compared to WT controls (P<0.05). g. Representative staining for MMP-9 sections from the outflow vein removed from Iex-1 KO (second column) and WT controls (third column) are shown. IgG antibody staining was performed to serve as negative control in the first column. Brown staining cells are positive for MMP-9. h. Pooled data from the semiquantitative analysis for intensity of MMP-9 staining in the vessel wall of the outflow vein specimens removed from Iex-1 KO and WT mice. There is a significant decrease in the mean MMP-9 staining in the Iex-1 KO animals when compared to WT controls (P<0.05). For all representative sections * denotes vessel lumen. 40X magnification, scale bar is 100-µms.