Figure 4. Human macrophage endogenous production of 13R,14S-diHDHA.

(A) MRM chromatography for ion pair 359>221 (m/z). Human macrophages were incubated with opsonized zymosan (DPBS+/+, pH 7.45, 15 min, 37°C). Incubations were stopped with 2 volumes of ice-cold methanol, and products were assessed by lipid mediator metabololipidomics. (B) MS-MS spectra. Inset, diagnostic ions used for identification and corresponding possessed structure fragments. Results are representative of n = 3.