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. 2014 Jul 18;9(7):e102797. doi: 10.1371/journal.pone.0102797

Figure 3. Effect of methylation at two CpG loci upstream of TATA-box on MeCP2 binding.

Figure 3

In vitro binding of nuclear protein from HeLa cells to TATA-box and two CpG dinucleotides upstream of TATA-box was tested by EMSA. (A) Double-stranded oligonucleotides, unmethylated (UUS/UUA), single-methylated (UMS/UMA or MUS/MUA), and double-methylated (MMS/MMA) were subjected to the binding reaction. (B) Both single-methylated and double-methylated oligonucleotides showed prominent protein-DNA binding at a high position (black arrowheads, lanes 3, 5, and 7), which was block-shifted by the anti-MeCP2 antibody (lanes 4, 6, and 8). The sequence date has been quoted from GenBank (accession number AF364906.1) by Wong VK et al [41].