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. 2014 Jul 18;9(7):e102957. doi: 10.1371/journal.pone.0102957

Figure 5. Impaired global sumoylation enhances Ago2 protein levels in cells and in mice.

Figure 5

(A) Depletion of SUMO enzymes results in increased steady-state Ago2 protein levels. HeLa cells were transfected with control siRNA (scr) or siRNA against Ubc9, SAE2 or RanBP2. Relative levels of Ago2 are indicated in the graph. Means of three independent experiments. Significant p-values are shown. (B) Loss of Ubc9 in vivo results in enhanced endogenous Ago2 protein levels. Ubc9+/+/ROSA26-CreERT2 control (Ubc9+/+) and Ubc9fl/−/ROSA26-CreERT2 conditional knockout (Ubc9−/−) adult mice (n = 3, #1, #2, #3) received 3 consecutive days of 4-OHT injection. Proteins were extracted from heart, liver and skeletal muscle and were analyzed by Western blot. Quantification of Ago2 protein levels in organs after normalization to the ponceau staining is shown. Bars represent means and standard deviations. from 3 mice (white bars: Ubc9+/+, black bars: Ubc9−/−). P-values, determined by two-tailed Student’s t-test, are indicated.