Fig. 2.
E2 regulation of PTEN is ERα-dependent. a Western blot analysis of whole cell lysates from ISH-ERαWT and ISH-ERαNEG cells with antibodies specific to ERα, ERβ, GPER, and GAPDH. b ISH-ERαNEG PTEN+ cells were starved in serum free media for 24 h followed by E2 treatment for 0, 10, 30, or 50 min. ISH-ERαNEG PTEN+ cells and ISH-ERαNEG empty vector control cells (EV) were harvested and analyzed via Western blotting with an antibody specific to phospho-PTEN (S380, T382, and T383) or c total PTEN. Corresponding graphs to the right represent quantification of fold change ± SD (n = 5) of a phospho-PTEN or b total PTEN normalized to GAPDH (P = ns, by ANOVA)