Fig. 5.
ERα and CK2α exist in a complex in both absence and presence of E2, while ERα and PTEN form a complex in an E2-dependent manner. a ISH-ERαWT PTEN+ and c ISH-ERαΔNLS PTEN+ cells were starved in serum free media for 24 h followed by E2 treatment for 0, 10, 30, or 50 min. Cells were harvested in nondenaturing buffer followed by immunoprecipitation (IP) with an antibody specific to ERα or IgG control antibody. Immunoprecipitates were analyzed via Western blotting with an antibody specific to ERα. Co-immunoprecipitation (co-IP) of CK2α was assessed with an antibody specific to CK2α. IgG arrow shows heavy chain IgG from ERα IP antibody (50 kDa). Representative Western blot images for a ISH-ERαWT PTEN+ cells and c ISH-ERαΔNLS PTEN+ cells shown (n = 3). b ISH-ERαWT PTEN+ and d ISH-ERαΔNLS PTEN+ cells were starved in serum free media for 24 h followed by E2 treatment and IP with an antibody specific to PTEN or IgG control antibody. Immunoprecipitates were analyzed via Western blotting with an antibody specific to PTEN and co-IP of ERα was assessed with an antibody specific to ERα. IgG arrow shows heavy chain IgG from PTEN IP antibody (50 kDa). Representative Western blot images for b ISH-ERαWT PTEN+ cells and d ISH-ERαΔNLS PTEN+ cells shown (n = 3)