Table 1.

Oligonucleotide sequences of primers for real-time RT-PCR

Target cDNA	GenBank acc no	Forward primers (5′ to 3′)	Reverse primers (5′ to 3′)	Amplicon size (bp)
BDNF 1–9	EF125675	tgttggggagacgagatttt	cgtggacgtttgcttctttc	159
BDNF 2a–9	EF125676	tacttcatccagttccaccag	caagttgccttgtccgt	129
BDNF 2b–9	EF125677	aagctccggttccaccag	tgcttctttcatgggcg	102
BDNF 2c–9	EF125678	gtggtgtaagccgcaaaga	cgtggacgtttgcttctttc	124
BDNF 3–9	EF125686	ctgagactgcgctccactc	gtggacgtttgcttctttca	152
BDNF 4–9	EF125679	gagcagctgccttgatgttt	gtggacgtttgcttctttca	148
BDNF 5–9	EF125687	accccgcacactctgtgta	acagctgggtaggccaagtt	204
BDNF 6–9	EF125680	gatccgagagctttgtgtgg	gtggacgtttgcttctttca	130
BDNF 8–9	EF125689	cagtggagctgaacaaacga	gccttcatgcaaccgaagta	117
BDNF 9a–9	EF125690	gtctctgcttccttcccaca	cgtggacgtttgcttctttc	124
Total BDNF	NM_012513	gcggcagataaaaagactgc	gcagccttccttcgtgtaac	141
TrkA	NM_021589	ttctcaagtgggagctaggg	ctctgcctcacgatggaagt	155
RPL27	NM_022514	gaattgaccgctatcccaga	tcgctcctcaaacttgacct	200

Notes: The forward primer for each BDNF splice variant is designed from each non-coding exon. The forward primer for total BDNF is designed from exon 9. All reverse primers except TrkA and RPL27 are designed from exon 9.

Abbreviations: BDNF, brain-derived neurotrophic factor; RPL27, 60S ribosomal protein L27; RT-PCR, reverse transcription (RT)-polymerase chain reaction; TrkA, tropomyosin receptor kinase A; bp, base pairs.