Table 1.

Mutations found in the cloning experiments of low activity NColE7 mutant genes

Multiplicity of mutations	Mutation
Single site	G473S, V476A, E488G, K525E, S535P, R538G, E542G, L543P, H545Y, D557E, D559 V, D559G, V564A, K567R
Double mutation	S474P + E508K F489L + S535P N560D + R568G
Triple mutation	G473C + E488D + L543P
Reading frame shift	IDIHRGK-LIFTEVNSS GK-VNSSSGRIVTD R574Stop

Single site mutations are point mutations, that occur along with the activity-decreasing designed N-terminal mutations, while double mutation sites occurred in pairs. Reading frame shifts are caused by the missing of one base. For more details see Table S4