Table 1.
Primers used for real time PCR: list of primers of internal control, early and late osteoclast markers and their sequences
| Primer | Gene name | Accession number | Sequence (5′→3′) | Size (bp) |
|---|---|---|---|---|
| Vitronectin | Vitronectin (Vtn), | NM_011707 |
Fwd: TGCAGCGTTCGCCCTTCCTG Rev: CCTCCTGGCTGGGTTGCTGC |
110 |
| Cathepsin K | Cathepsin K(Ctsk) | NM_007802 |
Fwd: CGTGCAGCAGAACGGAGGCA Rev: TAGCTGCCTTTGCCGTGGCG |
95 |
| Calcitonin receptor | Calcitonin receptor (Calcr), | NM_007588 |
Fwd: ACATGATCCAGTTCACCAGGCAGA Rev: AGGTTCTTGGTGACCTCCCAACTT |
107 |
| MMP9 | Matrix metallo-proteinase 9 (MMP9) | NM_013599 |
Fwd: TGAACAAGGTGGACCATGAGGTGA Rev: TAGAGACTTGCACTGCACGGTTGA |
121 |
| Carbonic anhydrase II | Carbonic anhydrase II | M81022 |
Fwd: ACCACTCCGCCTCTGCTGGA Rev: ACGCCAGTTGTCCACCATCGC |
144 |
| 18S | 18S ribosomal RNA (Rn18 s) | NR_003278 |
Fwd: AGTGCGGGTCATAAGCTTGC Rev: GGGCCTCACTAAACCATCCA |
134 |