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. 2014 Feb 11;27(4):351–360. doi: 10.1007/s40620-014-0054-3

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© The Author(s) 2014

Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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Fig. 3 — HCaRG stimulates renal cell migration. a Wound healing assay in Neo- or HCaRG-plasmid-transfected HEK-293 cells. HCaRG markedly accelerated wound healing after 20 h compared to Neo-controls. Scale bars 50 μm. b Intracellular location of F-actin (green) and HCaRG (red) in migrating MDCK cells during the wound-healing process. In non-migrating cells, HCaRG is located in the perinuclear space, and organized actin fibers are clearly visible. In the migrating cell, intracellular actin fibers are de-polymerized and short F-actin filaments are observed in the perinuclear space. HCaRG localizes with short F-actin filaments between the nucleus and elongated tip. Nuclei are stained by DAPI (blue). Scale bars 25 μm (color figure online)