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. 2014 Jul 17;55(2):227–237. doi: 10.1016/j.molcel.2014.05.025

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© 2014 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

RNF126 Associates with Bag6 via Its Ubl Domain

(A) Lysates from HEK293T cells transfected with either Flag-Bag6 or Flag-ΔUbl-Bag6 (input) were bound and eluted from Flag resin and the indicated fractions analyzed by SDS-PAGE and Coomassie staining.

(B) The fractions from (A) were immunoblotted for the indicated proteins. Asterisk denotes nonspecific band.

(C) The indicated proteins were coexpressed in HEK293T cells and analyzed by immunoblotting directly (input) or after IP with anti-HA antibodies (HA-IP).

(D) Summary of interactions between Bag6 and various RNF126 constructs. Numbers refer to amino acid residues on RNF126.

See also Figure S3.