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(A–F) J-factor curves from cyclization experiments. Experimental J-factor data (open symbols) for natural DNA (circles), diaminopurine substitution (triangles), and inosine substitution (diamonds) are shown as well as WLC fits (lines) and associated fit parameters: persistence length (P), helical repeat (γ0), and torsional modulus (C). Two different restriction sites, NarI ends (long dash) and HindIII ends (two dash), are shown for natural DNA along with the pooled data set (solid).
