Table 1.

Cyclization J-factor determined from kinetic rates k_C1 and k_D for the indicated DNA lengths

DNA variant	DNA length (bp)	J-factor (nM)	kC1 (× 10−3 min−1)	kD (× 10−3 nM−1 min−1)
Natural (NarI)	201	5.4 ± 1.3 (5.1 ± 1.2)	5.0 ± 1.5	1.0 ± 0.4
	202	3.0 ± 0.1 (2.8 ± 0.4)	4.7 ± 2.6	1.6 ± 0.9
	203	1.6 ± 0.3 (1.4 ± 0.3)	2.7 ± 0.5	1.8 ± 0.8
	204	0.7 ± 0.3 (0.8 ± 0.3)	1.0 ± 1.0	1.3 ± 0.6
	205	0.5 ± 0.1	0.4 ± 0.2	0.9 ± 0.3
	206	0.7 ± 0.1	1.2 ± 0.6	1.8 ± 1.0
	207	2.3 ± 0.4	2.7 ± 1.1	1.2 ± 0.5
	208	6.2 ± 1.4	4.2 ± 0.6	0.7 ± 0.1
	209	6.9 ± 1.5	3.1 ± 1.0	0.4 ± 0.1
	210	9.7 ± 2.4	5.3 ± 1.5	0.6 ± 0.2
	211	8.1 ± 3.3	7.4 ± 1.9	1.0 ± 0.2
Natural (HindIII)	196	1.1 ± 0.3	9.0 ± 4.5	7.6 ± 2.9
	197	2.0 ± 0.4	19.6 ± 9.2	10.0 ± 4.0
	198	3.1 ± 0.4	34.1 ± 14.0	10.9 ± 3.7
	199	4.1 ± 0.8	44.1 ± 14.6	10.6 ± 2.2
	200	4.6 ± 0.5	47.8 ± 9.1	10.3 ± 0.9
	201	4.6 ± 1.0 (5.1 ± 1.2)	43.6 ± 7.7	9.5 ± 0.8
	202	2.6 ± 0.5 (2.8 ± 0.4)	28.2 ± 2.8	11.2 ± 3.1
	203	1.3 ± 0.2 (1.4 ± 0.3)	13.8 ± 2.9	11.2 ± 2.3
	204	0.9 ± 0.2 (0.8 ± 0.3)	9.9 ± 2.3	11.5 ± 2.1
Diaminopurine	201	3.2 ± 0.1	1.1 ± 0.2	0.3 ± 0.1
	202	2.6 ± 0.9	1.3 ± 0.1	0.5 ± 0.2
	203	1.6 ± 0.3	1.1 ± 0.7	0.8 ± 0.6
	204	0.5 ± 0.1	0.7 ± 0.1	1.5 ± 0.1
	205	0.3 ± 0.1	0.3 ± 0.1	0.9 ± 0.6
	206	0.4 ± 0.1	0.2 ± 0.1	0.6 ± 0.3
	207	0.7 ± 0.1	0.5 ± 0.1	0.7 ± 0.1
	208	1.6 ± 0.2	1.5 ± 0.2	0.9 ± 0.2
	209	3.5 ± 0.6	4.4 ± 0.3	1.3 ± 0.1
	210	6.1 ± 1.2	6.7 ± 0.8	1.1 ± 0.1
	211	6.5 ± 0.8	4.1 ± 0.8	0.6 ± 0.1
Inosine	196	5.8 ± 1.6	17.9 ± 8.2	3.0 ± 0.5
	197	6.0 ± 3.2	8.1 ± 9.0	1.1 ± 0.7
	198	5.4 ± 0.7	6.5 ± 0.8	1.2 ± 0.2
	199	2.8 ± 0.3	3.5 ± 1.7	1.3 ± 0.6
	200	1.6 ± 0.2	3.7 ± 1.1	2.3 ± 0.7
	201	0.8 ± 0.1	3.1 ± 0.6	3.8 ± 0.7
	202	0.6 ± 0.1	2.2 ± 1.4	3.6 ± 1.9
	203	1.0 ± 0.2	4.8 ± 1.0	4.9 ± 0.5
	204	2.3 ± 0.2	2.9 ± 1.2	1.2 ± 0.4

Values are presented as mean ± standard deviation. Pooled J-factor data for natural DNA are indicated in parentheses.