Table 2.
Parameters determined from WLC analysis of cyclization data
| DNA variant | P (nm) | γ0 (bases/turn) | C (× 10−19 erg-cm) | Pt (nm) |
|---|---|---|---|---|
| Natural (NarI) | 47.4 ± 0.4 | 10.51 ± 0.01 | 2.20 ± 0.19 | 53.9 ± 4.7 |
| Natural (HindIII) | 48.1 ± 0.2 | 10.50 ± 0.01 | 1.84 ± 0.21 | 45.1 ± 5.2 |
| Natural (pooled) | 47.8 ± 0.3 | 10.51 ± 0.01 | 2.03 ± 0.13 | 49.8 ± 3.2 |
| Diaminopurine | 49.4 ± 0.4 | 10.54 ± 0.01 | 2.39 ± 0.22 | 58.7 ± 5.5 |
| Inosine | 47.1 ± 0.3 | 10.35 ± 0.01 | 1.86 ± 0.13 | 45.6 ± 3.1 |
Persistence length (P), helical repeat (γ0), and torsional rigidity (C) along with the related twist persistence length (Pt) are presented as mean ± standard deviation from Monte Carlo simulations.