Figure 5.

Screening of 38 Chlamydomonas strains for motility-deficient mutants using the CLONA method. Scatter plots of beat frequency f versus average swimming velocity $\overline{V}$ of the beat cycles. (Blue contour lines) The characteristic cluster of the control. The difference between roc30 and wild-type and the difference between roc105 and wild-type are statistically significant (assuming Wilks’s lambda distribution, p < 0.001). (Each dot) One beat cycle. Focus index > 80. (A) A scatter plot of roc55, a representative strain showing no deficiency in motility. (B) A scatter plot of roc30 (ODA4), a representative strain showing severe defects in motility. (C) A scatter plot of roc105, a representative strain of newly detected motility deficient mutants, showing mild deficiency in average swimming speed and beat frequency.