Table 4.
Reactions that were identified as irreversible by thermodynamic constraints in Yeast 5 that were not previously specified, or were specified with the opposite direction in the model
| ID | Reaction | ΔrGmin (KJ/mol) | ΔrGmax (KJ/mol) | Direction | Pathway | ID hGeM |
|---|---|---|---|---|---|---|
| r_1871 | [m]1p3h5c + h + h2o = 4hglusa | 41.44 | 98.52 | Reverse | Arginine and proline metabolism | PHCHGSm |
| r_1887 | [c]glu5sa = 1pyr5c + h + h2o | −88.7 | −31.62 | Forward | Arginine and proline metabolism | G5SADs |
| r_1888 | [m]glu5sa = 1pyr5c + h + h2o | −90.98 | −33.9 | Forward | Arginine and proline metabolism | G5SADrm |
| r_0483 | [c] (2)gthrd + h2o2 = gthox + (2)h2o | −316.29 | −202.13 | Forward | Glutathione metabolism | GTHP |
| r_0484 | [m] (2)gthrd + h2o2 = gthox + (2)h2o | −316.29 | −202.13 | Forward | Glutathione metabolism | GTHPm |
| r_0356 | [c]13dpg = 23dpg + h | −71.96 | −14.88 | Forward | Glycolysis/gluconeogenesis | DPGM |
| r_0911 | [c]air + co2tot = 5aizc + h + h2o | 3.34 | 71.84 | Reverse | IMP biosynthesis | AIRCr |
| r_0073 | [c]4ppmip + h2o = (2)h + minohp + pi | −69.16 | −0.67 | Forward | Inositol phosphate metabolism | NA |
| r_0092 | [c]6ppmip + h2o = (2)h + minohp + pi | −69.16 | −0.67 | Forward | Inositol phosphate metabolism | NA |
| r_0767 | [n]h2o + NAD = ADP-rib + h + ncam | −89.44 | −3.82 | Forward | NAD metabolism | NADN |
| r_0204 | [c]ap4a + h2o = (2)adp + (2)h | −102.97 | −17.35 | Forward | Nucleotides metabolism | ∼AP4AH1 |
| r_0326 | [c]dcmp + h + h2o = dump + nh4 | −90.02 | −4.4 | Forward | Pyrimidine catabolism | DCMPDA |
| r_0110 | [c]ac + coa + h = accoa + h2o | 15.52 | 101.14 | Reverse | Pyruvate metabolism | ACOAHi |
| r_0953 | [c]nh4 + (0.5)o2 + pydx + (2)h2o = (2)h2o2 + pydam | 306.21 | 454.62 | Reverse | Vitamin B6 metabolism | NA |
| r_0955 | [c]o2 + pdx5p = h2o2 + pydx5p | −162.89 | −65.86 | Forward | Vitamin B6 metabolism | PDX5PO |
Reactions in bold are those specified with the opposite direction in the model. See the Supporting Material for analysis of errors in estimated values. NA, not applicable.