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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Hepatol. 2014 Apr 21;61(2):358–365. doi: 10.1016/j.jhep.2014.04.009

Fig. 3. Statins and PLIN5 control LD contents in hepatocytes.

Fig. 3

(A–D) Mouse primary hepatocytes were incubated in media supplemented with BSA or BSA:oleate, in the presence or absence of 5 μmol/L simvastatin. The statin decreased TG accumulation, as measured by oil red O staining (A) and enzymatically (B). The relative mRNA (C) and protein (D) expression of hepatic perilipins in oleate-challenged hepatocytes was analysed in a parallel set of cells. Data are shown as mean ± SEM of 2 independent experiments in triplicate or quadruplicate, and were analysed by Student’s t-test. **P≤0.01, oleate vs. BSA. P≤0.01, SMV vs. DMSO. (E–G) siRNA transfection of mouse primary hepatocytes showed that dose-dependent decrease in PLIN5 expression (E) was paralleled by a concomitant decrease in TG contents as measured enzymatically (F) and by oil red O staining (G). Data in F are mean ± SEM of 2 independent experiments in triplicate, and were analysed by one-way ANOVA and post hoc Tukey’s test. *P<0.05; **P<0.01