Fig. 4. Plin5 reverses the effects of statins on TG contents and fatty acid oxidation.

Mouse primary hepatocytes were transduced with an empty or a Myc-Plin5 adenovirus, and then incubated in media supplemented with DMSO or 5 μmol/L simvastatin (SMV). (A) Immunoblots of selected hepatic proteins. (B) Intracellular TG contents, as determined enzymatically. Data are shown as mean ± SEM of 2 independent experiments in triplicate, analysed by two-way ANOVA followed by Mann-Whitney U-test. *P≤0.05 SMV vs. DMSO; ^¶P≤0.05, Adeno-Plin5 vs. Adeno-empty. (C) Representative oil Red O staining. (D) Fatty acid oxidation capacity was assessed in cells incubated with [³H]-palmitate, by measuring the release of [³H]-water. (E) De novo TG synthesis was assessed in cells incubated with [¹⁴C]-acetate (see Methods). Data in D and E are shown as mean ± SEM of 2 independent experiments in triplicate, and were analysed by two-way ANOVA followed by Mann-Whitney U-test. *P≤0.05 SMV vs. DMSO; ^¶P≤0.05, Adeno-Plin5 vs. Adeno-empty.