Table 1.
Averaged peak retention times and abundances for peaks that were common to both 24 and 48 hour infection samples, with distinguishable control and virus strains. (p < 0.05)
| Peak | Cntrl | MOI 1 H1N1 | MOI 10 H1N1 | MOI 10 H6N2 | MOI 10 H9N2 | Mutually distinguishable viral strains[a] |
Tentative compound (alternative(s)) |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| tR | A. | tR | A. | tR | A. | tR | A. | tR | A. | |||
| C1 | 1.92 (0.12) |
10047 (3800) |
2.05 (0.04) |
18975 (5286) |
2.07 (0.01) |
34638 (7187) |
2.06 (0.01) |
53071 (6207) |
2.07 (0.00) |
27768 (4821) |
Cntrl/MOI 1 H1N1; Cntrl/MOI 10 H1N1; Cntrl/MOI 10 H6N2; Cntrl/MOI 10 H9N2; MOI 1 H1N1/MOI 10 H1N1; MOI 1 H1N1/MOI 10 H6N2; MOI 1 H1N1/MOI 10 H9N2; MOI 10 H1N1/MOI 10 H6N2; MOI 10 H1N1/MOI 10 H9N2; MOI 10 H6N2/MOI 10 H9N2 | 2-methoxy-ethanol |
| C2 | – | – | 5.96 (0.09) |
109624 (136496) |
6.09 (0.07) |
15356 (13469) |
6.11 (0.09) |
15183 (8325) |
6.06 (0.09) |
14263 (4506) |
MOI 1 H1N1/MOI 10 H1N1; MOI 1 H1N1/MOI 10 H6N2; MOI 1 H1N1/MOI 10 H9N2 | thiirane |
| C3 | 12.62 (0.08) |
8601 (2233) |
12.62 (0.03) |
18629 (7844) |
12.71 (0.03) |
17975 (4401) |
12.69 (0.03) |
30312 (12957) |
12.71 (0.03) |
15104 (5906) |
Cntrl/MOI 1 H1N1; Cntrl/MOI 10 H1N1; Cntrl/MOI 10 H6N2; Cntrl/MOI 10 H9N2; MOI 1 H1N1/MOI 10 H6N2; MOI 10 H1N1/MOI 10 H6N2; MOI 10 H6N2/MOI 10 H9N2 | propanoic acid, ethyl ester |
| C4 | 19.71 (0.07) |
10925 (2735) |
19.75 (0.05) |
11214 (1939) |
19.79 (0.09) |
8924 (3672) |
19.85 (0.07) |
8271 (3269) |
19.87 (0.04) |
7127 (1269) |
Cntrl/MOI 10 H6N2; Cntrl/MOI 10 H9N2; MOI 1 H1N1/MOI 10 H6N2; MOI 1 H1N1/MOI 10 H9N2 | butanoic acid, 2-methyl-, methyl ester |
| C5 | 30.13 (0.04) |
10292 (3091) |
30.11 (0.06) |
18579 (7496) |
30.26 (0.04) |
13955 (3882) |
30.25 (0.04) |
23767 (13960) |
30.26 (0.04) |
10047 (2748) |
Cntrl/MOI 1 H1N1; Cntrl/MOI 10 H1N1; Cntrl/MOI 10 H6N2; MOI 1 H1N1/MOI 10 H9N2; MOI 10 H1N1/MOI 10 H6N2; MOI 10 H1N1/MOI 10 H9N2; MOI 10 H6N2/MOI 10 H9N2 | butanoic acid, 2-methyl-, ethyl ester |
| C6 | – | – | 35.43 (0.06) |
9945 (1756) |
35.59 (0.03) |
6493 (772) |
35.59 (0.04) |
7526 (2329) |
35.58 (0.04) |
6829 (1452) |
MOI 1 H1N1/MOI 10 H1N1; MOI 1 H1N1/MOI 10 H6N2; MOI 1 H1N1/MOI 10 H9N2 | hexan-3-one, 5-methyl- |
| C7 | – | – | 36.06 (0.11) |
7882 (842) |
36.25 (0.05) |
6463 (778) |
36.27 (0.05) |
6569 (1613) |
36.24 (0.05) |
5955 (528) |
MOI 1 H1N1/MOI 10 H1N1; MOI 1 H1N1/MOI 10 H6N2; MOI 1 H1N1/MOI 10 H9N2 | heptan-3-one |
| C8 | 48.10 (0.08) |
10895 (5148) |
48.10 (0.09) |
9703 (2285) |
48.16 (0.05) |
13743 (6077) |
48.17 (0.07) |
13705 (4113) |
48.15 (0.04) |
13617 (3724) |
MOI 1 H1N1/MOI 10 H1N1; MOI 1 H1N1/MOI 10 H6N2; MOI 1 H1N1/MOI 10 H9N2 | octan-2-one |
| C9 | 67.17 (0.05) |
22904 (3975) |
67.20 (0.06) |
22780 (3323) |
67.34 (0.04) |
14834 (4705) |
67.35 (0.04) |
14875 (3667) |
67.33 (0.04) |
13788 (2653) |
Cntrl/MOI 10 H1N1; Cntrl/MOI 10 H6N2; Cntrl/MOI 10 H9N2; MOI 1 H1N1/MOI 10 H1N1; MOI 1 H1N1/MOI 10 H6N2; MOI 1 H1N1/MOI 10 H9N2 | not identified |
| C10 | 68.59 (0.06) |
18440 (4064) |
68.59 (0.06) |
17319 (3374) |
68.76 (0.04) |
11818 (3835) |
68.77 (0.05) |
11820 (2142) |
68.76 (0.03) |
11314 (2118) |
Cntrl/MOI 10 H1N1; Cntrl/MOI 10 H6N2; Cntrl/MOI 10 H9N2; MOI 1 H1N1/MOI 10 H1N1; MOI 1 H1N1/MOI 10 H6N2; MOI 1 H1N1/MOI 10 H9N2 | 1-phenylbut-1-ene (1-ethenyl-4-ethyl-ben-zene, 1-ethenyl-3-ethyl-benzene, 1-methyl-4-(prop-2-enyl)-benzene) |
| C11 | 70.92 (0.06) |
9138 (2726) |
70.92 (0.07) |
8959 (1387) |
71.11 (0.03) |
7200 (1726) |
71.12 (0.04) |
7978 (1823) |
71.11 (0.05) |
7729 (1855) |
Cntrl/MOI 10 H1N1; MOI 1 H1N1/ MOI 10 H1N1 | 1-phenylbut-1-ene (1-ethenyl-4-ethyl-ben-zene, 1-ethenyl-3-ethyl-benzene, 1-methyl-4-(prop-2-enyl)-benzene) |
| C12 | 78.22 (0.07) |
133058 (594423) |
78.23 (0.07) |
1231871 (331605) |
78.41 (0.03) |
541882 (84018) |
78.41 (0.03) |
607416 (206791) |
78.40 (0.03) |
631543 (202320) |
Cntrl/MOI 10 H1N1; Cntrl/MOI 10 H6N2; Cntrl/MOI 10 H9N2; MOI 1 H1N1/MOI 10 H1N1; MOI 1 H1N1/MOI 10 H6N2; MOI 1 H1N1/MOI 10 H9N2 | 3,7-dimethyloctan-3-ol |
| C13 | 79.81 (0.06) |
15112 (4098) |
79.82 (0.07) |
13800 (2201) |
80.02 (0.04) |
11344 (8837) |
80.02 (0.03) |
9559 (1669) |
80.00 (0.03) |
9119 (1420) |
MOI 1 H1N1/MOI 10 H1N1; MOI 1 H1N1/MOI 10 H6N2; MOI 1 H1N1/MOI 10 H9N2 | 4-ethylbenzaldehyde |
| C14 | 88.63 (0.07) |
15356 (23190) |
88.61 (0.04) |
7988 (2512) |
88.78 (0.14) |
28348 (15442) |
88.82 (0.10) |
23635 (13835) |
88.87 (0.04) |
18743 (6348) |
MOI 1 H1N1/MOI 10 H1N1; MOI1 H1N1/MOI 10 H6N2; MOI1 H1N1/ MOI 10 H9N2 | decanal (isomers, homologues) |
tR: retention time [min]; A: abundance (total ion count); standard deviation in parentheses.
[a]
Student’s t-test (p ≤ 0.05).