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. Author manuscript; available in PMC: 2014 Jul 21.
Published in final edited form as: Mol Pharm. 2011 Aug 24;8(5):1582–1591. doi: 10.1021/mp200171d

Figure 2.

Figure 2

Polyplex activity, distribution inside hydrogel scaffolds and release. (A) Activity of the entrapped polyplexes was determined through the release of the polyplexes post hydrogel formation using trypsin and a subsequent bolus transfection with the released polyplexes. The gene transfer of the released polyplexes was compared to fresh polyplexes with trypsin added and fresh polyplexes with gel degradation products added. (B) DNA/PEI polyplexes were stained with ethidium bromide post hydrogel formation and imaged with a fluorescence microscope equipped with z-stack capability. Scale bar = 100µm. (C) DNA release was determined using radiolabeled DNA. DNA/PEI loaded hydrogels were incubated in different release solutions and at predetermined time points samples were gathered and analyzed for radioactivity using a scintillation counter. At the final day of the release assay the hydrogel was fully degraded with trypsin and the final activity measured. Data is plotted as the % cumulative release.