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. Author manuscript; available in PMC: 2014 Jul 21.
Published in final edited form as: Angew Chem Int Ed Engl. 2011 Jan 26;50(10):2289–2293. doi: 10.1002/anie.201006523

Figure 3.

Figure 3

System performance in 12-plex assay. (A) Calibration curves for particle batches, with background-subtracted signal plotted against spiked target amount. miR-210, −221, −222 and let-7a were spiked into the same incubation mixes at the indicated amounts. The remaining seven naturally-occurring targets (‘+’ symbols) were spiked into the 27- and 243-amol trials to validate performance. For all trials, 200 ng of E. coli total RNA was also spiked in for complexity. Mean CV of target level is 6.35% when considering target levels greater than 5 amol. Each point represents, on average, 19 particles from a single run. (B) Specificity of let-7a probe in the presence of sequences closely related to intended target. (C) Cancer profiling results for dysregulated targets in four human tissue types. Error bars represent standard deviation in triplicate measurements on aliquots of the same single-patient sample. Amount of total RNA used in gel-particle assays is 250 ng, unless otherwise noted. See supporting information for details of dysregulation analysis.