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. Author manuscript; available in PMC: 2015 Apr 18.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Mar 22;446(4):1120–1125. doi: 10.1016/j.bbrc.2014.03.077

Fig. 1.

Fig. 1

TALE-SIDs that bind AXIN2 associate with HDAC1 and repress AXIN2-luciferase reporter activity. (A) Schematic of the AXIN2 gene locus with the transcription start site on the right side of the figure. Grey rectangles indicate the ß-catenin-bound regions identified in a β-catenin ChIP-Seq screen [15]. A diagram of the TALE-SIDs is boxed below and shown is their target sequence within the first exon of AXIN2. (B) Western blot analysis of protein lysates prepared from HEK293 cells transfected with the indicated FLAG-tagged TALE constructs and immunoprecipitated with anti-FLAG antibodies. (C) Luciferase reporter assays in HEK293 cells transfected with a pGL3-basic plasmid that contains a 787-bp fragment of the AXIN2 gene encompassing the TALE binding sites inserted upstream of the luciferase gene. Where indicated, cells were co-transfected with plasmids encoding β-cateninS45F, LEF, TALE1- SID, and TALE2-SID. Error is SEM (* P < 0.05). (D) Luciferase reporter assays in HEK293 cells transfected with the indicated pGL3 luciferase plasmids and plasmids encoding TALE-SIDs.