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. Author manuscript; available in PMC: 2015 Jul 3.
Published in final edited form as: Mol Cell. 2014 Jun 19;55(1):148–155. doi: 10.1016/j.molcel.2014.05.017

Figure 3. 2AP Fluorescence Lifetimes Reveal the Structural Context of the tRNA 3′ End Bound to the T-box Antiterminator.

Figure 3

(A) Normalized traces of 2AP fluorescence decay and best fits for RNAs in (B). IRF: instrument response function.

(B) Secondary structures of RNAs analyzed in (A). Scissors indicate nick position in the split T-box reconstituted with T-box170 and T-box171–182.

(C) Average 2AP fluorescence lifetimes of the RNAs shown in (B), as colored in (A). See also Figure S3. Error bars represent s.d. from three independent experimental replicates.