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. 2014 Jun 13;11:111. doi: 10.1186/1743-422X-11-111

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Copyright © 2014 Carter et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Schematic of the group I (trans-splicing) intron expression cassette. Each trans-splicing αDENV-U143 group I intron was tagged downstream of the ΔN Bax 3’ exon with the mCherry fluorescent marker gene expressed from the Drosophila C virus (DCV) IRES sequence. Expression of this construct was driven by the Drosophila melanogaster Actin 5c promoter (A5c). This bi-cistronic configuration allowed monitoring for the presence and expression of the anti-DENV group I intron constructs within cell cultures. A5c = Drosophila Actin 5c promoter, EGS = External guide sequence, IGS = Internal guide sequence, TSD = Trans-splicing domain, ΔN Bax = pro-apoptotic 3’ exon, IRES = Internal ribosome entry site.