Figure 3.

Assessment of ΔN Bax expression and proapoptotic activity. A. Western blots were performed as described in Methods. DCA, DCA-ΔN Bax, FMDV2A-ΔN Bax, and ΔN Bax probed with Bax C-terminal specific antibody. The bottom bands are corresponding actin loading controls. B. The relative amount of amido black recovered from each well is inversely proportional to the extent of apoptosis. The DCA wells were set to a value of 1 for each time point and the readings for all other wells at each time point were adjusted by the corresponding ratio [50]. Measurements were recorded in triplicate for each time point. Statistical analysis was performed using ANOVA test with Tukey post test. Differences were statistically significant with p values less than 0.05.