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. 2014 Jun 13;11:111. doi: 10.1186/1743-422X-11-111

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Copyright © 2014 Carter et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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DNA Fragmentation Assay: Clonal Aedes albopictus C6/ 36 cells transformed with αDENV -U143-FL, αDENV -ΔU143-ΔN Bax or αDENV-U143-ΔN Bax were each challenged with one of four known DENV serotypes (MOI 0.1). At 4 dpi (for DENV) cells were pelleted, lysed, and analyzed as described in Methods. Uninfected clonal and wild type C6/36 cells were also assayed as an additional negative control.