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. Author manuscript; available in PMC: 2015 Jun 5.
Published in final edited form as: Mol Cell. 2014 May 22;54(5):820–831. doi: 10.1016/j.molcel.2014.04.026

Figure 5. Overexpression of Non-degradable HIF-1α Mutant Rescues ER Stress-mediated Apoptosis in Cancer Cells with Reduced OGT In Vitro.

Figure 5

(A) Cell lysates were collected from MDA-MB-231 cells overexpressing control or HIF-1αP402A, P546A and either infected with control or OGT-1 shRNA. Cells were lysed and proteins were analyzed by immunobloting. (B) MDA-MB-231 cells were treated with control or OGT shRNA after stable infection with control or HIF-1αP402A, P546A. Cell colonies were stained with crystal violet. (C) MDA-MB-231 cells as in (B) were analyzed for apoptosis after lentiviral infection with control or OGT shRNA. Cells were stained with Annexin V/PI and analyzed by flow cytometry. * p-value<0.05.