Preadipocytes were stimulated with TNF-α (1 nM), IL1-β (1 nM), and IL-6 (50 ng/ml) with soluble receptor (100 ng/ml) for 24 hours. Upon harvest, RNA was isolated from preadipocytes, reverse transcribed and evaluated by quantitative real-time PCR.
Results (mean ± SEM, n = 3–4) are expressed relative to control (represented by the dotted line).
*P < 0.05.