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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: J Endocrinol. 2014 Apr 29;222(1):87–97. doi: 10.1530/JOE-13-0610

Figure 4. Efficacy of inhibitor concentrations.

Figure 4

Human preadipocytes from omental fat were treated with BAY11–7082 (5 μM), SP600125 (20 μM) or SB203580 (2.5 μM) and cytokines TNF- α (1nM) and IL-1β (1 nM) for 6 hours prior to cell lysis for Western Blotting. (A) Effect of BAY11–7082 on cytokine-induced NFκB activation (IκBα phosphorylation and degradation). (B) Effect of SP600125 on cytokine-induced JNK activation (phosphorylation of JNK). (C) Effect of SB203580 on cytokine-induced p38 kinase activation (phosphorylation of Hsp27). C = Control.