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. Author manuscript; available in PMC: 2014 Nov 9.
Published in final edited form as: Science. 2014 May 5;344(6184):649–652. doi: 10.1126/science.1251152

Figure 1. Rejuvenation of muscle stem cells by heterochronic parabiosis.

Figure 1

(A). Frequency of clone-sorted satellite cells from isochronic (Iso) or heterochronic (Het) mice forming colonies after 5 days in culture. All colonies showed characteristic morphology of muscle lineage cells. (B) DNA damage in freshly sorted satellite cells assessed by single cell gel electrophoresis under alkaline conditions. Damage was quantified using a visual scoring metric (25) (key at top) and represented by color-coding: no damage (green), moderate damage (orange), maximal damage (red). (C) Representative images (confocal z-stacks) of freshly sorted satellite cells stained with DAPI (blue) and anti-pH2AX (green); data are quantified in (D). All graphs represent mean ± SD, with p-values, calculated by Mann-Whitney analysis. “n=” indicates number of mice used for each analysis. Scale bar = 10µm.