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. Author manuscript; available in PMC: 2014 Nov 9.
Published in final edited form as: Science. 2014 May 5;344(6184):649–652. doi: 10.1126/science.1251152

Figure 2. Rejuvenation of muscle stem cells by rGDF11 supplementation.

Figure 2

(A, B). Frequency (A) and myogenic colony formation (B) of satellite cells from vehicle- or rGDF11-treated mice. (C). Quantification of DNA damage assays using freshly sorted satellite cells from vehicle- or rGDF11-treated mice, scored as in Fig. 1B. (D, E). H&E staining (D) and frequency distribution of myofiber size (E) in regenerating TA muscles 7 days after cryoinjury in vehicle- or rGDF11-treated young and aged mice. Scale bar = 100µm. (F). Representative images of transverse cryosections of TA muscles 2 weeks after transplantation. (G) Quantification of transplant data as maximal number of GFP+ myofibers found in each engrafted muscle. Graphs represent mean ± SD (in A–C, G). p-values were calculated by Mann-Whitney analysis (A–C), Wilcoxon Exact analysis (E), or Student’s t-test (G). “n=” indicates number of mice used for each analysis.