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. 2014 Jul 15;5:4420. doi: 10.1038/ncomms5420

Figure 1. CFTR Cl− currents in mouse pancreatic islet β-cells and its activation by glucose.

Figure 1

(a,b) Whole-cell Cl− currents recorded with CsCl pipette solution in CFTR wild-type (a) or DF508 mutant (b) β-cells before (control) and 5 min after the addition of forskolin (10 μM) and 3 min after CFTR inhibitor glyH-101 (10 μM) with corresponding I-V curves (n=3). (c) Whole-cell Cl− current recorded with NMDG-Cl pipette and bath solution in wild-type mouse β-cells before (control) and 10 min after the addition of glucose (10 mM), and subsequently 3 min after glyH-101 (10 μM) with corresponding I-V curves (n=3). Pulse protocol: 20 mV steps from −100 mV to +100 mV for 100 ms. Extracellular Cl− concentration ([Cl−]o)=142 mM; Intracellular Cl− concentration ( [Cl−]i )=150 mM; equilibrium potential for Cl− (ECl)=1.4 mV. Data are shown as mean±s.e.m.