Figure 4. Involvement of CFTR in glucose-induced insulin secretion.

(a) Effect of CFTRinh-172 and glyH-101 on glucose-induced insulin secretion of islets isolated from mice. CFTRinh-172 (10 μM) and glyH-101 (10 μM) significantly reduced insulin secretion 5, 15 and 60 min after glucose (10 mM) challenge. The experiment was repeated three times. *P<0.05, ***P<0.001, one-way analysis of variance. (b) Reduced glucose (10 mM) -induced insulin secretion from CFTR DF508 islets as compared with the wild-type. n=3–4, *P<0.05, t-test. (c,d) Time-course change in blood glucose (c) and insulin (d) after glucose injection (intraperitoneal 2 g kg⁻¹ body weight) in DF508 and the wild-type mice. *P<0.05; **P<0.01. n=6, t-test. Data are shown as mean±s.e.m.