Figure 5. Rescue of DF508 defects by corrector VX-809.

Effect of VX-809, a corrector of DF508 CFTR, in rescuing DF508 defects, resting membrane potential (a, by patch-clamp), Ca²⁺ response (b, by Fura-2) and insulin secretion (c). Islets/β-cells isolated from DF508 mice were pretreated with VX-809 (10 μM) for 48 h before the experiments. DMSO (0.1% v/v) was also applied to both wild-type and DF508 islets/β-cells as vehicle control for comparison. Number of measurements is shown in each column. *P<0.05,**P<0.01, one-way analysis of variance. Data are shown as mean±s.e.m.