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| kcat (s−1) | KM NO2− (mM) | kcat/KM (mM−1 s−1) | |
|---|---|---|---|
| Wild-type AxNiR* | 89±3 | 0.027±0.005 | 3,300 |
| F306C | 310±10 | 1.2±0.1 | 260 |
NO2, nitrite.
*Data obtained from ref. 7. Values shown are the mean±s.d. of two individual experiments. The apparent kinetic constants for both the wild-type and F306C summarized here were obtained by nonlinear fitting of the data.
