Figure 8. Effect of miR-9 inhibitor on LPS-induced microglial activation in vivo.

(a) Effect of anti-miR-9 on microgliosis induced by LPS in the SNr. Representative immunostained image for Iba-1 in SNr of mice injected with saline/LPS. Scale bar, 200 μm (upper panel); 100 μm (middle panel); 50 μm (lower panel). Stereology of Iba-1-positive microglia in SNr. All the data are indicated as mean±s.d. N=4 animals per group. *P<0.05 versus anti-miR group treated with saline, ^#P<0.05 versus anti-miR-control treated with LPS using one-way analysis of variance followed by Holm–Sidak test. (b) Effect of anti-miR-9 on the CD11b+/CD45 dim population of microglia isolated from mouse brain by Percoll gradient method and assessed by flow cytometric analysis. (c) Effect of LPS on the expression of miR-9 in microglia isolated by Percoll gradient method. Mice were injected with LPS (1 mg kg⁻¹, i.p.) for 5 days. Total RNA isolated from microglia was subjected to real-time PCR analysis using primer sets specific for mature miR-9. All the data are indicated as mean±s.d. N=10 animals per group. *P<0.05 versus saline group using Student’s t-test.