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. 2014 Jul 9;5:4340. doi: 10.1038/ncomms5340

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(a) Structural variations in the GmCHX1 coding region of salt-sensitive germplasms, with salt-tolerant W05 as comparison. (b) SNP analyses and multiple amino-acid sequence alignments of the GmCHX1 coding region from different soybean germplasms. X denotes ambiguous amino acid due to low coverage of re-sequencing data. Black indicates conserved residues. (c) SNP analyses and multiple alignments of a 2-Kb promoter region of GmCHX1 from different soybean germplasms. Conserved bases are highlighted in black. R=G/A, Y=T/C. (d) Expression study of GmCHX1 in soybean germplasms. Real-time PCR of the GmCHX1 gene using primers specific to regions upstream (upper panel; for tolerant lines and C08) or downstream (lower panel; for all germplasms) referenced to the retrotransposon insertion site in C08, using RNA from salt-tolerant (green bars) and salt-sensitive germplasms (blue bars). N=3. Error bar=s.e.m.