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. 2014 Jul 9;5:4340. doi: 10.1038/ncomms5340

Figure 5. Gain-of-function analysis of GmCHX1 using transgenic tobacco BY-2 cells.

Figure 5

(a) Trypan blue staining of tobacco BY-2 cells under NaCl treatment. Four-day-old cells were treated with 100 mM NaCl or remained untreated in MS medium for 20 h before staining with Trypan blue. Nuclei of dead cells were stained blue. Scale bars, 100 μm. (b) Calculated survival rate based on the results of Trypan blue staining. The data were calculated from 14 randomly taken photos for each sample. (c) Na+/K+ ratio of BY-2 cells under NaCl treatment. BY-2 cells were treated with 100 mM NaCl or remained untreated in MS medium for 4 h. Ion contents were determined using atomic absorption spectrophotometry. N=4. ND, non-detectable due to low signals. Numerical data in b and c were analysed using one-way analysis of variance followed by the Tukey’s post hoc test (P<0.05). Error bars=s.e.m. Line no. 8 and Line no. 9 are two independent transgenic lines of GmCHX1. WT, untransformed wild type; and GFP, BY-2 cells transformed with the GFP gene as a negative control.