Figure 11.
Capsular and decapsular portion after the enucleation of the adrenal gland. The enucleation of the adrenal gland was performed using rats fed on Na-deficient diet for 20 days in order to detect zG clearly as CYP11B2-expressing zone. After the enucleation, the capsular and the decapsular portion were stained using anti-rat CYP11B2 and anti-rat CYP11B1 antibodies simultaneously. A: The capsular portion of the adrenal gland. Note that, on the plate, a section of the capsular portion is bent, and one inner edge of zU is located side by side with another edge of zU. B: The decapsular portion of the adrenal gland. In both figures, blue and brown colors show the presence of CYP11B2 and CYP11B1, respectively. Nuclei were poststained with methyl green. zG: zona glomerulosa, zF: zona fasciculata, zR: zona reticularis, zU: undifferentiated cell zone. Refer to data from Mitani et al.100) and Mitani and Ishimura.101)