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. Author manuscript; available in PMC: 2014 Jul 21.
Published in final edited form as: Methods Mol Biol. 2012;794:187–197. doi: 10.1007/978-1-61779-331-8_11

Figure 1.

Figure 1

Schematic diagram showing the DNA components described in this protocol with nucleotides numbered relative to original transposon insertion site. A) The cloning site of pIT-X indicating correct reading frame. Oligonucleotides used for protein of interest must match the correct reading frame. B) Mly I transposon showing positioning of restriction sites as it would insert into a segment of DNA. C) Reading-frame selectable linker showing restriction sites, frame, and amber stop codon scar in bold.