Table 1. Activity of CaM-affinity purified ACA12.
Ca2+-ATPase activity of ACA12 was measured as described in the Materials and Methods. Results (plus or minus standard error of the mean) are from three experiments, each with three replicates, performed on different purified fractions.
Assay conditions | Ca2+-ATPase activity (nmol Pi min−1 mg−1 protein) |
---|---|
ATP | 289 ± 27 |
ATP + 1 µM CaM | 303 ± 31 |
ATP + 10 µM CaM | 309 ± 29 |
ATP + 200 µM vanadate | 3 ± 1 |
ITP | 25 ± 3 |